| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Emerald Force Soccer Club - 01 Emerald Force 07 Atletico |
0214-011B4U-4580 | Adelmo Santos |
| A2 | Tennessee Soccer Club TSC 2007 Boys ECNL |
0522-011B4U-0081 | Gregory Warden |
| A3 | Tennessee United Soccer Club TUSC 2007 Boys Red |
0424-011B4U-0032 | Eduardo Carvacho |
| A4 | Tennessee Soccer Club TSC 2007 Boys Nike |
0522-011B4U-0080 | Jordan Grant |
| A5 | Lobos Rush (Collierville Soccer Association) 07 Lobos Rush Memphis Blues |
0603-011B4U-0002 | Russell Church |
| A6 | Nashville United Soccer Academy - 01 NUSA B07 Maroon |
0534-011B4U-0008 | Giles Cheevers |
| A7 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 07B SL |
0324-021B4U-0009 | Christopher Zeller |
| A8 | Aris FC Aris FC - B14U Black 2007 |
0530-022B4U-0104 | Brian Davies |